|Year : 2016 | Volume
| Issue : 3 | Page : 190-193
Effect of automated red cell exchanges on oxygen saturation on-air, blood parameters and length of hospitalization in sickle cell disease patients with acute chest syndrome
John C Aneke1, Nancy Huntley2, John Porter2, Perla Eleftheriou2
1 Department of Haematology, Nnamdi Azikiwe University Teaching Hospital, Nnewi, Nigeria; Department of Haematology, Red Cell Unit, University College London Hospital, London, UK
2 Department of Haematology, Red Cell Unit, University College London Hospital, London, UK
|Date of Web Publication||15-Jun-2016|
John C Aneke
Department of Haematology, Nnamdi Azikiwe University Teaching Hospital, PMB 5025, Nnewi, Anambra, Nigeria
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: Red cell exchanges (RCEs) lead to improvement in tissue oxygenation and reduction in inflammatory markers in sickle cell disease (SCD) patients who present with acute chest syndrome (ACS). The aim of this study is to evaluate the effects of automated-RCE (auto-RCE) on oxygen saturation (SpO 2 ) on-air, blood counts, the time to correct the parameters and length of hospitalization after the exchange in SCD patients presenting with ACS. Subjects and Methods: This was 2 years study involving five SCD patients; the time for SpO 2 on air to increase to ≥95% and chest symptoms to resolve, postprocedure, as well as the length of in-patient hospitalization was recorded. All data were entered into Statistical Package for Social Sciences Version 20.0 (SPSS Inc., Chicago, IL, USA) computer software for analyses. Results: The study involved 4 (80%) hemoglobin (Hb) SS and 1 (20%) HbSC patients. The median time of SpO 2 recovery was 24 h, ranging from 6 to 96 h. About 60% (3/5) of patients achieved optimal SpO 2 within 24 h post-RCE, while discharge from intensive care unit was 24 h after auto-RCE in one patient. The Hb concentration was significantly higher, while the total white cell and absolute neutrophil counts were significantly lower at the time of resolution of symptoms, compared to before auto-RCE (P < 0.05). The average post auto-red cell transfusion symptoms duration was 105.6 (24-240) h while mean inpatient stay was 244.8 (144-456) h. Conclusion: Auto-RCE could reverse hypoxia in ACS within 24 h.
Keywords: Acute chest syndrome, auto-red cell exchange, oxygen saturation, sickle cell disease
|How to cite this article:|
Aneke JC, Huntley N, Porter J, Eleftheriou P. Effect of automated red cell exchanges on oxygen saturation on-air, blood parameters and length of hospitalization in sickle cell disease patients with acute chest syndrome. Niger Med J 2016;57:190-3
|How to cite this URL:|
Aneke JC, Huntley N, Porter J, Eleftheriou P. Effect of automated red cell exchanges on oxygen saturation on-air, blood parameters and length of hospitalization in sickle cell disease patients with acute chest syndrome. Niger Med J [serial online] 2016 [cited 2021 Jun 13];57:190-3. Available from: https://www.nigeriamedj.com/text.asp?2016/57/3/190/184073
| Introduction|| |
Sickle cell disease (SCD) is an autosomal recessive genetic disorder in which there is the homozygous inheritance of the sickle hemoglobin (HbS) or in association with any other abnormal Hb.  The hallmarks of this disorder include recurrent acute events known as crises which interrupts steady chronic anemia. Repeated crises and persistent anemia have been shown to predispose to significant end-organ dysfunction, particularly in the homozygous (HbSS) state of the disease. , Sickle red cells when exposed to reduced oxygen tension assume an abnormal, rigid (sickle) shape that adhere together with platelets and white blood cells (WBCs) to the endothelial wall, leading to vascular occlusion, ischemia, and endothelial dysfunction. ,
Acute chest syndrome (ACS) is a potentially fatal pulmonary complication of SCD, characterized by intravascular pulmonary red cell sickling. The clinical spectrum spans from a mild pneumonic illness to life-threatening acute respiratory distress syndrome and multi-organ failure. It is commonly heralded by a fall in alveolar oxygen tension, leading to polymerization of sickle red cells, vaso-occlusion, decreased pulmonary blood flow, and hypoxia. Cytokines, such as vascular cell adhesion molecule 1 are critical in the development of ACS, as it enhances the adherence of red cells to the pulmonary endothelial cells, thus exacerbating vaso-occlusion and hypoxia.  Some identified risk factors for the development of this condition include splenectomy,  abdominal surgery,  asthma,  infection,  fat emboli, and sickle vaso-occlusive pain. 
If untreated, severe cases of ACS may progress to persistent hypoxemia and hypercapnia, resulting in respiratory failure and invariably death.  It has been estimated that up to 50% of patients with SCD could have at least one episode of ACS in their lifetime. 
Red cell exchange (RCE) (erythrocytapheresis) is an effective important component of the management of sickle-related complications.  Common indications include ACS, stroke, multi-organ failure syndrome, and priapism. , RCE is superior to simple transfusion in that it reduces the percentage of circulating sickle cells while increasing the red cell oxygen carrying capacity, without causing a corresponding increase in blood viscosity and body iron. Increased blood viscosity alters blood rheology and predisposes to ischemic adverse events such as vaso-occlusive crises and stroke while iron overload causes marked organ tissue damage, with an increase in disease morbidity and mortality. ,,,
Documented effects of RCE in the setting of ACS include improved tissue oxygenation, changes in Hb oxygen affinity and blood oxygen pressure, increase in transcutaneous oxygen saturation (SpO 2 ), increased HbA content with reduced HbS fraction and reduced WBC count and inflammatory markers. ,,,,
There is a paucity of literature on the timelines for resolution of parameters (such as SpO 2 and blood counts) in SCD patients with ACS, who had received RCE, particularly automated-RCE (auto-RCE). The aim of this study was, therefore, to evaluate the spectrum of changes in the blood counts and SpO 2 in SCD patients with ACS, post auto-RCE, with a view to highlighting changes in counts at symptom resolution and the timelines for saturation to become optimized.
| Subjects and methods|| |
This was a retrospective study, carried out at the red cell unit of the University College London Hospital, involving confirmed SCD patients aged ≥18 years who presented to the emergency room with ACS between the months of January 2014 and January 2016. Diagnostic features considered in keeping with ACS included fever, cough, chest pain, shortness of breath, reduced SpO 2 , hemoptysis, and suggestive chest X-ray findings with or without preceding history of acute pain crisis.  Other inclusion criteria were hospitalization on account of ACS ≥24 h and at least one session of auto-RCE in the index admission.
The data of individual patients were retrieved from the departmental paper medical notes as well as the hospital's patient data program. Sociodemographic information, date and time of admission, symptoms at presentation, date and time of auto-RCE procedures, serial recordings of SpO 2 on room air (from the point of admission till resolution of chest symptoms and discharge from hospital), time of resolution of chest symptoms post-RCE and length of hospitalization were extracted. The results of serial blood counts: WBC count, Hb concentration, platelet count, absolute neutrophil count (ANC), absolute lymphocyte count, platelet count and the neutrophil-lymphocyte ratio (NLR) were similarly retrieved from the electronic patient data program of the unit. All data were entered into Statistical Package for Social Sciences version 20.0 (SPSS Inc., Chicago, IL, USA) computer software for further analyses.
The time for SpO 2 (in room air) to increase to ≥95%,  was noted for each patient, and this was taken as the time for significant improvement in SpO 2 to have taken place, post auto-RCE. The means of blood counts before auto-RCE were compared with values recorded at the point of symptom resolution using the Student's t-test while the level of statistical significance was set at P ≤ 0.05 (at 95% confidence interval).
| Results|| |
A total of 5 patients, with mean age of 40.40 ± 5.12 years, including 4 HbSS and 1 HbSC; two males and three females were studied.
The mean of Hb concentration was significantly higher in all patients at the point of resolution of symptoms compared to the value recorded before auto-RCE (105.40 ± 7.90 g/L vs. 81. 80 ± 8.53,P = 0.01, [Table 1]).
|Table 1: Comparison of means and standard deviation of haematological indices before automated red cell exchange and at symptom resolution |
Click here to view
Correspondingly, the means of total white and ANCs were significantly lower at the point of symptom resolution in all patients, compared with values before auto-RCE (9.47 ± 3.52 × 10  /L vs. 14.03 ± 2.41 × 10  /L; 5.21 ± 3.44 × 10  /L vs. 8.26 ± 4.88 × 10  /L, P values 0.03 and 0.04, respectively, [Table 1]).
The means of platelet count and NLR were lower at the point of symptom resolution in all patients compared with values before auto-RCE. However, these differences were not statistically significant (P > 0.05, [Table 1]).
The median time of SpO 2 recovery in all patients was 24 h (6-96 h); 60% (3/5) of all patients achieved optimal SpO 2 within 24 h post auto-RCE, whereas 80% (4/5) achieved this within 48 h. Only one patient was admitted to the intensive care unit (ICU) at presentation; discharge from ICU occurred 24 h after auto-RCE.
By the time of symptom resolution, the WCC and platelet counts had returned to normal in 60% (3/5) of study patients. The average duration of chest symptoms, post auto-RCE, was 105.6 (24-240) h while mean in-patient stay was 244.8 (144-456) h.
| Discussion|| |
The median time for SpO 2 to increase to ≥95% post auto-RCE in this study was 24 h. The SpO 2 cut-off of ≥95% was chosen as it conforms to the British Committee for Standards in Haematology guidelines on ACS; it is believed to represent the point of optimal oxygen-carrying capacity of the red cells.  Increasing hypoxemia and hypercapnia have been shown to be important hallmarks of ACS and in severe cases can progress to death from acute hypoxic respiratory failure.  Auto-RCE for ACS with HbAA red cells can provide a rapid and dramatic improvement in oxygen carrying capacity which reverses hypoxaemia and hypercapnia, commonly reflected as an increase in SpO 2 .  The timelines required for this to take place has however not been sufficiently studied. 
In this study, 60% (3/5) and 80% (4/5) of patients achieved optimal tissue oxygenation in 24 h and 48 h, post auto-RCE, respectively. The above timelines demonstrated in this study could well indicate the times expected to achieve management goals in patients with ACS on auto-RCE. Physicians could, therefore, find this information handy in making management decisions as well as during counseling of patients/relatives on clinical recovery and possible length of hospital stay. Further study of larger subjects is suggested to confirm our finding and generate more data on auto-RCE in SCD patients with ACS. Interestingly, one of the patients was observed to have achieved optimal SpO 2 (96%) by 96 h post auto-RCE but desaturated over the next 5-9 days, subsequently stabilizing over the next 11-12 days. It is important to note that persistent desaturation post-RCE could, in fact, be a "red flag" for further investigation for documented conditions such as unresolved chest infection, and other co-morbidities such as asthma, pulmonary hypertension and the presence of chronic sickle lung disease.  These conditions influence SpO 2 and may require specific interventions as applicable. The index patient had underlying pulmonary hypertension secondary to past episodes of ACS, which probably explains the pattern of SpO 2 observed.
In this study, we observed that the WBC and ANC showed a uniform decrease in patients post auto-RCE and remained significantly lower at the point of resolution of symptoms compared to the values obtained before the exchange. Our finding is in agreement with the earlier report of Marques et al., who observed a reduction in WBC count post-RCE.  This could be as a result of the reported post-RCE blunting of inflammation;  white cells, particularly neutrophils tend to increase in inflammation, as part of the acute phase response.  The Hb concentration increased progressively in all the patients, postprocedure and remained significantly higher than preexchange levels at the point of symptom resolution, this is consistent with the documented increase in Hb content which occurs post-RCE. 
The average length of inpatient hospitalization in this study is similar to that reported by Vichinsky et al. in adults patients (10.2 days vs. 10.5 days, respectively) but higher than observed by Sprinkle et al. in children (10.2 days vs. 7 days, respectively). , The average time for resolution of chest symptoms post auto-RCE (4.4 days) was shorter than the total inpatient hospitalization time in this study. Some of our patients had extended hospital stay due to concurrent co-morbidities such as renal impairment and pulmonary hypertension. It is, therefore, likely that the in-patient hospitalization, post auto-RCE could be similar to that observed in children (and may parallel the time of resolution of chest symptoms), in the absence of significant co-morbidities. There is a need to investigate for concurrent co-morbidities in SCD patients with ACS, who may need extended hospitalization post auto-RCE.
Limitations of this study
The small sample size could have affected the power of our conclusions.
| Conclusion|| |
Auto-RCE continues to show tremendous benefit in the setting of ACS, particularly with regards to prompt restoration of SpO 2 and timely resolution of presenting chest symptoms. It, therefore, has remarkable potential in significantly reducing lengths of ICU and hospital stay in SCD patients with this life-threatening complication.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Lal A, Vichinsky EP. Sickle cell disease. In: Hoffbrand AV, Catovsky D, Tuddenhan EG, editors. Postgraduate Hematology. 5 th
ed. Oxford: Blackwell Publishing; 2005. p. 104.
Aneke JC, Adegoke AO, Oyekunle AA, Osho PO, Sanusi AA, Okocha EC, et al.
Degrees of kidney disease in Nigerian adults with sickle-cell disease. Med Princ Pract 2014;23:271-4.
Okpala I. The intriguing contribution of white blood cells to sickle cell disease - A red cell disorder. Blood Rev 2004;18:65-73.
Stuart MJ, Setty BN. Sickle cell acute chest syndrome: Pathogenesis and rationale for treatment. Blood 1999;94:1555-60.
Ghantous S, Al Mulhim S, Al Faris N, Abushullaih B, Shalak F, Yazbeck S. Acute chest syndrome after splenectomy in children with sickle cell disease. J Pediatr Surg 2008;43:861-4.
Kokoska ER, West KW, Carney DE, Engum SE, Heiny ME, Rescorla FJ. Risk factors for acute chest syndrome in children with sickle cell disease undergoing abdominal surgery. J Pediatr Surg 2004;39:848-50.
Boyd JH, Macklin EA, Strunk RC, DeBaun MR. Asthma is associated with acute chest syndrome and pain in children with sickle cell anemia. Blood 2006;108:2923-7.
Neumayr L, Lennette E, Kelly D, Earles A, Embury S, Groncy P, et al.
Mycoplasma disease and acute chest syndrome in sickle cell disease. Pediatrics 2003;112(1 Pt 1):87-95.
Vichinsky EP, Neumayr LD, Earles AN, Williams R, Lennette ET, Dean D, et al.
Causes and outcomes of the acute chest syndrome in sickle cell disease. National Acute Chest Syndrome Study Group. N Engl J Med 2000;342:1855-65.
Castro O, Brambilla DJ, Thorington B, Reindorf CA, Scott RB, Gillette P, et al.
The acute chest syndrome in sickle cell disease: Incidence and risk factors. The Cooperative Study of Sickle Cell Disease. Blood 1994;84:643-9.
Swerdlow PS. Red cell exchange in sickle cell disease. Hematology Am Soc Hematol Educ Program 2006;2006:48-53.
Kim HC. Red cell exchange: Special focus on sickle cell disease. Hematology Am Soc Hematol Educ Program 2014;2014:450-6.
Thurston GB, Henderson NM, Jeng M. Effects of erythrocytapheresis transfusion on the viscoelasticity of sickle cell blood. Clin Hemorheol Microcirc 2004;30:83-97.
Thuret I. Post-transfusional iron overload in the haemoglobinopathies. C R Biol 2013;336:164-72.
Porter JB, Garbowski M. The pathophysiology of transfusional iron overload. Hematol Oncol Clin North Am 2014;28:683-701, vi.
Kim HC, Dugan NP, Silber JH, Martin MB, Schwartz E, Ohene-Frempong K, et al.
Erythrocytapheresis therapy to reduce iron overload in chronically transfused patients with sickle cell disease. Blood 1994;83:1136-42.
Emre U, Miller ST, Gutierez M, Steiner P, Rao SP, Rao M. Effect of transfusion in acute chest syndrome of sickle cell disease. J Pediatr 1995;127:901-4.
Uchida K, Rackoff WR, Ohene-Frempong K, Kim HC, Reilly MP, Asakura T. Effect of erythrocytapheresis on arterial oxygen saturation and hemoglobin oxygen affinity in patients with sickle cell disease. Am J Hematol 1998;59:5-8.
Nifong TP, Domen RE. Oxygen saturation and hemoglobin A content in patients with sickle cell disease undergoing erythrocytapheresis. Ther Apher 2002;6:390-3.
Liem RI, O'Gorman MR, Brown DL. Effect of red cell exchange transfusion on plasma levels of inflammatory mediators in sickle cell patients with acute chest syndrome. Am J Hematol 2004;76:19-25.
Marques MB, Singh N, Reddy VV. Out with the bad and in with the good; red cell exchange, white cell reduction, and platelet reduction. J Clin Apher 2014;29:220-7.
Howard J, Hart N, Roberts-Harewood M, Cummins M, Awogbade M, Davis B; BCSH Committee. Guideline on the management of acute chest syndrome in sickle cell disease. Br J Haematol 2015;169:492-505.
Turner JM, Kaplan JB, Cohen HW, Billett HH. Exchange versus simple transfusion for acute chest syndrome in sickle cell anemia adults. Transfusion 2009;49:863-8.
Ramaiah SK, Jaeschke H. Role of neutrophils in the pathogenesis of acute inflammatory liver injury. Toxicol Pathol 2007;35:757-66.
Sprinkle RH, Cole T, Smith S, Buchanan GR. Acute chest syndrome in children with sickle cell disease. A retrospective analysis of 100 hospitalized cases. Am J Pediatr Hematol Oncol 1986;8:105-10.
|This article has been cited by|
||Red cell exchange for patients with sickle cell disease: an international survey of current practices
| ||Matthew S. Karafin,Jeanne E. Hendrickson,Haewon C. Kim,Aisha Kuliya-Gwarzo,Monica B. Pagano,Ajay Perumbeti,Patricia A. Shi,Yvette C. Tanhehco,Jennifer Webb,Edward Wong,Quentin Eichbaum |
| ||Transfusion. 2020; |
|[Pubmed] | [DOI]|
||Guidelines on the Use of Therapeutic Apheresis in Clinical Practice – Evidence-Based Approach from the Writing Committee of the American Society for Apheresis: The Eighth Special Issue
| ||Anand Padmanabhan,Laura Connelly-Smith,Nicole Aqui,Rasheed A. Balogun,Reinhard Klingel,Erin Meyer,Huy P. Pham,Jennifer Schneiderman,Volker Witt,Yanyun Wu,Nicole D. Zantek,Nancy M. Dunbar,Guest Editor: Joseph Schwartz |
| ||Journal of Clinical Apheresis. 2019; 34(3): 171 |
|[Pubmed] | [DOI]|
||Use of red blood cell exchange for treating acute complications of sickle cell disease
| ||Ilknur Kozanoglu,Hakan Ozdogu |
| ||Transfusion and Apheresis Science. 2018; |
|[Pubmed] | [DOI]|
||Case series supporting heme detoxification via therapeutic plasma exchange in acute multiorgan failure syndrome resistant to red blood cell exchange in sickle cell disease
| ||James E. Louie,Caitlin J. Anderson,Katayoun Fayaz M. Fomani,Alonye Henry,Trevor Killeen,Narla Mohandas,Karina Yazdanbakhsh,John D. Belcher,Gregory M. Vercellotti,Patricia A. Shi |
| ||Transfusion. 2017; |
|[Pubmed] | [DOI]|