author = {Ajayi, O. and Ebeigbe, J.}, title = {{Plasmid profile of bacteria isolated from tears of HIV/AIDS patients}}, journal ={Nigerian Medical Journal}, volume ={50}, number ={1}, pages = {4-8}, year = {2009}, abstract ={Objective: The purpose of this study is to determine the presence and transfer of plasmids in bacteria isolated from tears of HIV/AIDS patients, their sensitivity and resistance to commercially available antibiotics. Design: This was a cross sectional experimental study. Materials and methods:One hundred tears samples from HIV/ AIDS patients and fifty tears samples from HIV/AIDS negative patients were screened for resistance to 14 commercially available antibiotics using disc diffusion method. Result: Three multiple antibiotics resistant strains of staphylococcus aureus and four multiple antibiotics resistance strains of Pseudomonas aeruginosa were identified. staphylococcus aureus strains showed 100% resistance to Ampiclox and erythromycin, 66.6% to Perfloxacin, amoxicillin and septrin, 33.33% to ciprofloxacin. Pseudomonas aeruginosa strains showed 100% resistance to streptomycin, amoxicillin, septrin and chloramphenicol. Only I strain of staphylococcus aureus showed presence of plasmid which was not transferable to Escherichia coli because of presence of disulphide cross-­linked cell wall. Other strains of both staphylococcus aureus and Pseudomonas aeruginosa remained resistant after curing . Conclusion: Further studies are needed in this area to show if antibiotic resistance in HIV/AIDS positive patients could be as a result of plasmid as well as other factors.}, URL ={https://www.nigeriamedj.com/article.asp?issn=0300-1652;year=2009;volume=50;issue=1;spage=4;epage=8;aulast=Ajayi;t=6}, eprint ={https://www.nigeriamedj.com/article.asp?issn=0300-1652;year=2009;volume=50;issue=1;spage=4;epage=8;aulast=Ajayi;t=6} }