Year : 2009 | Volume
: 50 | Issue : 1 | Page : 4--8
Plasmid profile of bacteria isolated from tears of HIV/AIDS patients
OB Ajayi, JA Ebeigbe
Department of Optometry, Faculty of Life Sciences, University of Benin, Nigeria
Objective: The purpose of this study is to determine the presence and transfer of plasmids in bacteria isolated from tears of HIV/AIDS patients, their sensitivity and resistance to commercially available antibiotics.
Design: This was a cross sectional experimental study.
Materials and methods:One hundred tears samples from HIV/ AIDS patients and fifty tears samples from HIV/AIDS negative patients were screened for resistance to 14 commercially available antibiotics using disc diffusion method.
Result: Three multiple antibiotics resistant strains of staphylococcus aureus and four multiple antibiotics resistance strains of Pseudomonas aeruginosa were identified. staphylococcus aureus strains showed 100% resistance to Ampiclox and erythromycin, 66.6% to Perfloxacin, amoxicillin and septrin, 33.33% to ciprofloxacin. Pseudomonas aeruginosa strains showed 100% resistance to streptomycin, amoxicillin, septrin and chloramphenicol. Only I strain of staphylococcus aureus showed presence of plasmid which was not transferable to Escherichia coli because of presence of disulphide cross-linked cell wall. Other strains of both staphylococcus aureus and Pseudomonas aeruginosa remained resistant after curing .
Conclusion: Further studies are needed in this area to show if antibiotic resistance in HIV/AIDS positive patients could be as a result of plasmid as well as other factors.
J A Ebeigbe
Department of Optometry, Faculty of Life Sciences, University of Benin
|How to cite this article:|
Ajayi O B, Ebeigbe J A. Plasmid profile of bacteria isolated from tears of HIV/AIDS patients.Niger Med J 2009;50:4-8
|How to cite this URL:|
Ajayi O B, Ebeigbe J A. Plasmid profile of bacteria isolated from tears of HIV/AIDS patients. Niger Med J [serial online] 2009 [cited 2020 Oct 30 ];50:4-8
Available from: https://www.nigeriamedj.com/article.asp?issn=0300-1652;year=2009;volume=50;issue=1;spage=4;epage=8;aulast=Ajayi;type=0